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The winning poster of 20th World Sterilization Congress

Celebrating Sterilization Science

We proudly congratulate the winning poster of the 20th World Sterilization Congress.


New technologies for improving Hygiene Control of borrowed instruments

Bronberg, Rosana1; Iemmello, Antonella1; Díaz, Juan2
1 Finochietto Hospital; 2 Terragene S.A.; 2 [email protected]


Assess the cleanliness of the Hospital’s borrowed surgical instruments, and to decrease the number of scheduled surgeries that are cancelled due to their dirtiness, by using new technologies for hygiene control and penalizations.


Each container as well as the most challenging instrument for cleaning inside it were visually examined using a magnifying glass and a gauze with alcohol.
The material that did not present visible dirtiness was analyzed through a surface protein quantification method Chemdye® PRO1 Micro along with Bionova® MiniPro Auto-reader incubators (Terragene®, Argentina).
The swabbing method and the acceptance limit (5 µg/instrument side) were taken from HTM 01-01 recommendations.
The results were traced through Bionova® Traceability Software.
A Dirtiness Rate (DR) was generated in order to perform a temporary statistical monitoring (dirt material/received material * 100).


– A total amount of 3348 containers were received.
219 were rejected due to visible dirt remains (N= 10; 22%) or to protein remains (N= 209; 78%) (Table 1).
– The DR was reduced in 49,6 % (from 9.32% to 4.62%), despite the amount of received material was almost doubled.
– One of the Institutions that included the quantification protein test as part of its washing control reduced its Dirtiness Rate from 26.47% to 0%.
– The surface protein quantification system allowed the detection of instruments with non-visible dirt.
– Traceability Software allows to have traceability over institutions responsible for the cleaning.

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